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Philips Healthcare rema assay
Rema Assay, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rema+assay/assay+rema/pmc12305296-151-9-28
Average 86 stars, based on 1 article reviews
rema assay - by Bioz Stars, 2026-11
86/100 stars

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Activity Assay:

Article Title: Repurposing of FDA ‐Approved Drugs to Disrupt Iron Uptake in Mycobacterium abscessus : Targeting Salicylate Synthase as a Novel Approach
Article Snippet: IC 50 was calculated by measuring enzymatic activity in the presence of different compound concentrations, using (Equation ), where A[I] is the enzyme activity at inhibitor concentration [I] and A[0] is the enzyme activity without inhibitor. (1) A I = A 0 × − I I + IC 50 The inhibition constant values ( K i ) were determined by assaying Mab ‐SaS at different substrate and compound concentrations and using an adapted equation for competitive inhibition (Equation ) with GraphPad 8.0 software. (2) v = V max S S + K m 1 + I K i To verify that the compounds are not PAINs, their ability to inhibit enzymatic activity was assayed in the presence of 0.1 mg/mL bovine serum albumin (BSA) or in the presence of 0.01% (v/v) Triton X‐100 to confirm that they did not promote protein aggregation, and in the presence of 100 mM of 1,4‐dithio‐DL‐threitol (DTT) to exclude reaction with cysteines (Mori et al. ). .. Antimycobacterial activity of the best compounds was evaluated by REMA assay in iron‐limiting chelated Sauton's medium on M. smegmatis MC 2 155 (Siegrist et al. ; Mehra and Philips ). ..



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